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TheTRPV1Agonist

Member Since 10 Sep 2017
Offline Last Active Today, 09:35 AM

#1499871 Can you help explain this stem narrowing/constriction?

Posted by TheTRPV1Agonist on 19 October 2017 - 09:45 PM

I was wondering if anyone has seen this before and could tell me what caused it and what the prognosis is for my little seedling. It's happened in more than one of my seedlings unfortunately. 

 

Here's the salient info: 

  • Narrowing occurred about 1cm (1/2 inch) above the soil line
  • The roots did look a bit like they might have started to rot 
  • There wasn't the best drainage in the container and the soil was pretty dense
  • I recently moved it outdoors, but was using a plastic tube to protect it from the wind. 
  • The plant itself looks very healthy, with no signs of yellowing, wilting, etc. 

 

The pepper itself is just a plain old cayenne, so not too worried if I lose it. 

 

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#1499653 Overwintered plant, before and after!

Posted by TheTRPV1Agonist on 18 October 2017 - 10:48 PM

Very glad to see it found its vigour! What is the time between the before and after?




#1499486 When to use initials and who decides - More Naming Convention

Posted by TheTRPV1Agonist on 18 October 2017 - 08:24 AM

I think the PL-B notation is a stretch. Isn't it just more appropriate to refer to it as the creator intended (i.e. brown bhutlah)? Unfortunately naming isn't easy, and even when there is a central body, new names or conventions are slow to be adopted. In medicine there's heaps of this, and it's always a conflict between the arguably easier to say eponymous name, or more descriptive, newer name. Ultimately you end up just having to learn both names so it really doesn't make things simpler. I wish I could offer an alternative, but I don't really have any immediate suggestions. It's got me thinking though. 




#1498132 Doubled Haploids (Anther Culture) Introduction to concept/possible at Home?

Posted by TheTRPV1Agonist on 12 October 2017 - 01:02 PM

 

I'm curious, what is the physical process/procedure for this? If you happen to know

 

I'm very new to this area so my understanding is limited. All I know is that a certain cocktail of chemicals can induce the pollen to form an embryo that will germinate. You can then induce aneuploidy, producing a diploid plant from your haploid with another chemical mediator (colchicine). As wiki puts it: 

 

"Since chromosome segregation is driven by microtubules, colchicine is also used for inducing polyploidy in plant cells during cellular division by inhibiting chromosome segregation during meiosis; half the resulting gametes, therefore, contain no chromosomes, while the other half contains double the usual number of chromosomes (i.e., diploid instead of haploid, as gametes usually are), and lead to embryos with double the usual number of chromosomes (i.e., tetraploid instead of diploid). While this would be fatal in most higher animal cells, in plant cells it is not only usually well tolerated, but also frequently results in larger, hardier, faster-growing, and in general more desirable plants than the normally diploid parents; for this reason, this type of genetic manipulation is frequently used in breeding plants commercially." 

 

https://en.wikipedia...e#Botanical_use

 

I hope that makes sense, happy to clarify if some of the terminology/concepts are not familiar. 




#1498118 Doubled Haploids (Anther Culture) Introduction to concept/possible at Home?

Posted by TheTRPV1Agonist on 12 October 2017 - 11:48 AM

Thanks Gorizza! Fortunately I've got access to all those articles via my university. I have read the last paper and also came across another (below) that is quite recent. Hopefully in the coming months I'll be able to evaluate each and see what's most feasible with my resources. Thanks for the extra reading, I'll let you know if I have any questions! 

 

Parra-Vega V., Seguí-Simarro J.M. (2016) Anther Culture in Pepper (Capsicum annuum L.). In: Germana M., Lambardi M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY




#1498068 Has anyone grown: 'Candy Cane' F1, 'Mad Hatter' F1 or 'Na...

Posted by TheTRPV1Agonist on 12 October 2017 - 06:33 AM

Would love to be kept posted on that Orange Viper.

 

Will do! Here's the only info I have on it. From a nursery in Australia called Haar's. Their website has nothing on their "Food For Life: Hot Heads" range. The SHU is oddly specific, but I guess if it is a new variety they're just going with the original as a guide? 

 

 

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#1498067 Doubled Haploids (Anther Culture) Introduction to concept/possible at Home?

Posted by TheTRPV1Agonist on 12 October 2017 - 06:27 AM

TL;DR

  • Cross plants to make a hybrid
  • Grow the F1 plant from the seed
  • Use fancy techniques to grow a plant from pollen alone
  • More fancy footwork - now your seed is stable, do with it as you please. 

Result: a stable cross within just two generations (rather than the 6,7,8) and arguably a more targeted approach bringing your wildest crosses to fruition. 

 

WARNING: somewhat technical
 
Preamble.... 

Despite this is only my first year growing, I've been swept away by the beauty and diversity of the Capsicum genus and I've spent these past several months intensely researching some of the more technical details of the genetics of these plants. I'm so enthralled and can't wait to develop some crosses of my own. Though I don't have a background in botany or horticulture, I do in biomedical science, and so I've had a bit of a leg up in starting to get my head around things.

 

While I plan to cross by hand, admittedly there's a lot in the way of producing a new, stable cultivar. Following the initial dihybrid cross (selfing of the F1), there's a lot of variation in the F2. Say you're interested in 3 gene loci (Aa, Bb, Cc), then it follows that only 1/64 of your F2 will actually be homozygous recessive at the 3 loci (aa, bb, cc). In order to get your desired cross, you have to grow out a lot of seed, and to stabilise you need to repeat the process for a few generations, tolerating some subtle and not so subtle variation (obviously not for the fully recessive plant...but anyway) Don't get me wrong, there are probably a lot of happy accidents that come from this process, but surely there's a more targeted way of getting your desired cross (cue anther culture) 

 

 

Doubled Haploids/Anther culture... 

 

So I've been reading up on a technique used commercially, which involves the usual cross between the two parents, and growing out of the F1 seeds, but following this, the anthers are taken and cultured in such a way that what you get are immediately stable (homozygous) without any further crossing. To explain this genetically, take the AA;BB;CC x aa;bb;cc cross. Your F1 is just Aa;Bb;Cc and then there are 8 different F2 genotypes as a result of random assortment. So your pollen might have the following A;B;C / a;B;C / A; b; c / and so on. What the technique involves is then taking the pollen (or anthers) from your F1s and culturing them in such a what that you actually get a plant that germinates from this culture without having gone through sexual reproduction. The resultant plant is a hemizygote, or haploid, that has only 1 set of genes, instead of the usual 2 sets. With a chemical such as colchicine you can then initiate doubling of the genome into 2 sets (or more...) Ultimately, you get a plant that bares fruits with seeds that are all homozygous, depending on the genotype of the pollen (i.e. A;B;c becomes AA; BB; cc or a;B;C becomes aa;BB;CC, etc) 

 

Now for my question ... has anyone got experience with Anther culture or related techniques and has actually ever tried this at home? If so, what protocol did you follow? 

 

Though there are some specialised chemicals/materials involved, a cursory search has found that they are both not excessively costly or illegal to possess. 

 

If anyone is interested in the protocols I've come across I can include some references! 

 

 

 

 

 

 




#1498061 Has anyone grown: 'Candy Cane' F1, 'Mad Hatter' F1 or 'Na...

Posted by TheTRPV1Agonist on 12 October 2017 - 05:11 AM

Thanks for your thoughts! I've actually got some Fish Peppers that I'm growing from seed, so I'm interested to see how crossing the Candy Cane works out. 

 

These last couple of purchases have admittedly been impulsive - but I've got pepper madness. Up to 30-odd varieties since starting indoors in May, we'll see how I manage as a first timer. 

 

Any idea how they manage to come up with hybrids at such a large scale? Presumably they can't just cross-pollenate all of them by hand? I'd imagine if you just left two parent plants in close proximity  to cross-pollinate then only some of the yielding seed would be crossed, with others being self-pollinated and resembling the parent.

 




#1498054 Baccatum with longest stem

Posted by TheTRPV1Agonist on 12 October 2017 - 03:01 AM

Yeah, it's a bit confusing but I don't know another word for it. I'm talking about the stalk from the plant to the fruit. 

 

That would be the Peduncle. http://www.chileplan...omy of chil.jpg




#1498049 Has anyone grown: 'Candy Cane' F1, 'Mad Hatter' F1 or 'Na...

Posted by TheTRPV1Agonist on 12 October 2017 - 01:30 AM

Hey there, 

 

I picked up these plants from a nursery (Bunning's, for my fellow Aussies) and was wondering if anyone here tried them this year, and if so, how did you find growing them? I believe the first two are new in 2017, and initially I thought they were just a Fish Pepper and a Bishop's Crown that had just been rebranded. Apparently they're both sweet peppers (so I feel somewhat swindled, but at least it should be fun to play around with in terms of cross-breeding/growing out the mystery bag that is saved F1 seeds) 

The Naga Viper Orange I couldn't find an info on anywhere either and wondering if it's just a red one or possibly a hybrid? 

 

Thanks in advance 

 

panamseed [dot] com/plant_info.aspx?phid=048300244004463 

panamseed [dot] com/plant_info.aspx?phid=048300001036968