pics Indoor Savina Plant on Steroids (tissue stock plants - pics)

[megahurts]

You can keep the cells dormant on a shelf until you're ready to grow the plant? That's a new one for me. What kit did you buy? This is real cloning. I guess this is the lab method for large horticultural producers of out of season plants.

Are you saying you can keep a collection of dormant samples in the mediums "on file"?

http://www.youtube.com/watch?v=kSiB4_zdQfM&feature=fvwrel

 

[mushroombob]

Yes, to a certain extent, you can keep back-up cultures of your plants.
many species, and selections within a species, will react differently to "in vitro" (meaning "in glass") culture techniques, but once figured out it can be a good way to propagate your plants if you have the know-how.

It is not too difficult to do, bbut it doesn't always make sense to do.

In my experience, mostly with non-succulent Euphobia spp. and papaya, you can leave a plant in a jar under light for weeks at a time, even months, with little problem. then when the plant starts to get too big or crowded, you open the jar, take out a couple shoot tips and put them in a new jar to start all over again.

Without liquid nitrogen, really long term storage where you dont have to interact with the plants would be difficult. even with liquid nitrogen, some plants might not like it. I have experimented with cultures under refrigeration a little, but it didnt work out very well. my plants looked pretty bad, and someone took the out and they died.

more later. I have to go. if anyone wants to discuss tissue culture further, should we start a thread? or does the op mind if we continue in this thread?

.

 

[GordonCheek]

I love it! Would like to see you do some thread or two on some of your work for shure. I am shure it would be quite educational. At least for me
Gordon.
I did do some work with orchid breeding in the distant past.
Great looking plant by the way!

 

[FarmerGuy]

Hijack away! The more knowledge out there the better.

The longest I've kept them is 4 months so far in the Unicorn jars once things transitioned to differentiated growth @ 60F., 65% RH and < 100 lumens. Those worked out just fine. In a couple months when I do my next run, I'm going to try and go a full year just to see what happens.

Yes, to a certain extent, you can keep back-up cultures of your plants.
many species, and selections within a species, will react differently to "in vitro" (meaning "in glass") culture techniques, but once figured out it can be a good way to propagate your plants if you have the know-how.

It is not too difficult to do, bbut it doesn't always make sense to do.

In my experience, mostly with non-succulent Euphobia spp. and papaya, you can leave a plant in a jar under light for weeks at a time, even months, with little problem. then when the plant starts to get too big or crowded, you open the jar, take out a couple shoot tips and put them in a new jar to start all over again.

Without liquid nitrogen, really long term storage where you dont have to interact with the plants would be difficult. even with liquid nitrogen, some plants might not like it. I have experimented with cultures under refrigeration a little, but it didnt work out very well. my plants looked pretty bad, and someone took the out and they died.

more later. I have to go. if anyone wants to discuss tissue culture further, should we start a thread? or does the op mind if we continue in this thread?

.