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micropropagation of Capsicum sp.

You shouldn't have too much trouble with it.

On one of our CP forums, beginners without science backgrounds have picked it up and successfully grown vfts and other CPs from seed and even a few were done with explant material.

It's a lot easier to grow things from seed in tc than it is to use explant material and of course sterile conditions are the key.

To get started, you can order a lot of the materials from Phytotech and there is even a Kitchen Tissue Culture web page that teaches the basics using household kitchen utensils and supplies.

dvg
 
Micropropagation or Tissue Culture (TC), is a way of taking plant material, either seeds or the growing point of a plant (meristem) or even a leaf in the case of a venus fly trap or a sundew and growing it on in sterile conditions inside a sealed glass jar or flask.

The trick is making sure that the plant material is entirely sterilized prior to putting it in the flask or all sorts of microbes and fungi will overtake the culture.

The advantage of TC is that from a single seed or appropriate piece of plant material, with the application of multiplying hormones, one can make millions of clones of a plant in a remarkably short period of time.

This is useful with very rare plants or even with award winning plants that a willing market is waiting to purchase, which is the case with orchid cultivars or even certain agricultural crops.

dvg
 
dvg said:
Micropropagation or Tissue Culture (TC), is a way of taking plant material, either seeds or the growing point of a plant (meristem) or even a leaf in the case of a venus fly trap or a sundew and growing it on in sterile conditions inside a sealed glass jar or flask.

The trick is making sure that the plant material is entirely sterilized prior to putting it in the flask or all sorts of microbes and fungi will overtake the culture.

The advantage of TC is that from a single seed or appropriate piece of plant material, with the application of multiplying hormones, one can make millions of clones of a plant in a remarkably short period of time.

This is useful with very rare plants or even with award winning plants that a willing market is waiting to purchase, which is the case with orchid cultivars or even certain agricultural crops.

dvg
Click to expand...

A tedious and artistic method for most growers, but in mycology I've isolated wild mycelium on agar before so it shouldn't be that hard with plant material. Something to note: 11/12 Dishes became contaminated...just to show you how hard it is.
 
I have a lot of experience with microprop. not with peppers, but with papaya and poinsettia (and a number of other Euphorbia spp)

I also have a lot of experience with mushroom cultivation.

There are a lot of similarities, the main point being sterile technique. if you master that, the rest is pretty easy.

Micropropagation is not necessarily applicable to every situation. it can be useful for wide crosses (interspecific hybrids that might not normally make it)

I have used it to produce hybrids in the genus Euporbia. after some time after pollination (can vary by species) the embryos are taken out and placed in a sterile nutrient gel and grown into little plants.

It does sound easier than it is, and it can take some time for things to get growing well in a petri dish, but if you get it right, it can be very useful.
 
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