Uh-Oh... Not sure without seeing the plant here in front of me, but this kinda looks like powdery or Downy mildew beginning. You can tell by looking for spores. They'll look like velvet on the effected area. If it's powdery, they will be on top. If it's downy, they will be on the bottom (of the leaves I mean). If it's just one leaf, remove it on a nice DRY day, not while it's raining. If all over, and you're not gonna eat the punkin, go to your local ag. supply place and get something to spray it with. If it is powdery mildew, it will defoliate your vine, and kill the plant.