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pics Indoor Savina Plant on Steroids (tissue stock plants - pics)

This plant orginated from the hottest Red Savina I ever grew. Thought I would let him make a couple peppers and went to work with a wet Q-tip 6 days ago. I use this plant and few others of different varieties (Scorp, Bih Jolokia, Bhut Jolokia, etc.) to keep consistentcy in my summer garden every year. This year is going quite well.

Check out the growth on this one. He just smells 'hot' when you rustle his leaves (only way I can describe it):

Pepper153.jpg




Pepper152.jpg


Pepper154.jpg
 
Farmer Guy,

How are you growing that plant? hydro? and how do you root your cuttings?

Thanks!
The plant looks great!


I'm calling it "sort of hydro" - the soilless media is in the little breathable tube and I water with nutrients I make here at home that seem to work really well. Usually water once every other day and that is it.

The plant itself is actually from tissue culture/clone, so it started life as a 1mm x 1mm piece of leaf and grew for ~4 weeks in a sterile jar with hormone-laced agar. then it went into a rockwool cube and 1 week later into that little tube. Right now it's getting 9 hours of light per day.
 
You grew this from a few cells of a tissue culture, wow. Did you buy one of those kits that come with jars, etc? Very interesting method. Why do this the mad-scientist way though and not use traditional cloning techniques from partial stems? Is there an advantage?
 
"hormone-laced agar" hmm, very interesting.

mind documenting the procedure next time you do it? I'm very interested to know your cloning process.
 
You grew this from a few cells of a tissue culture, wow. Did you buy one of those kits that come with jars, etc? Very interesting method. Why do this the mad-scientist way though and not use traditional cloning techniques from partial stems? Is there an advantage?

Yup, you can do it by regular cloning, but there are a couple drawbacks. Speed is one of them - I can get a lot more clones in a slightly longer period of time. Also, I can build up a clone stock and just let them sit for months in the jars with almost no light and cool temperatures which is something you can't do with regular clones. This fall, I'm going to pick the best-of-the-best plants from my garden and replicate those. That way I'll have an army of even better ones for next year.

Since everything happens in a sterile environment (like clean room sterile), I don't have to worry about bugs getting to them or things like bacterial spot no matter how long I plan on keeping them. Once the the sterile parts of the procedure is done and goes well, they require almost no care and can theoretically be kept at a non-growing pace indefinitely. I'm pretty new to this technique and have only done it with african violets, sempervivums, peppers and tomatoes. My goal is to be able to do it with bamboo, but I've got a ways to go until I can do that successfully.

In about 2-3 months I'll be trying it with some awesome Nardellos that came my way through the generosity of Justaguy (if you haven't tried his sauces BTW, they are awesome) who's also in central PA. When I do that I'll document the whole thing and take a bunch of pictures then put 'em up here.

Eventually, once I master bamboo, I'd like to move to the next level which is gene splicing. I'm at least 2 years off from that though, but you can do some pretty funny stuff with gene splicing - I guy I met at a midwest university made a corn plant that produced vanillin (vanilla) in the kernels. Imagine a spicy watermelon that comes off the vine! One could do all kinds of neat stuff (but not things that can kill the entire planet like Monsanto does, their GM products are very evil).
 
That is one amazing plant !!!
The whole process sounds very interesting. I'm looking forward to a tutorial on this subject.
Would you be willing to share your nute recipe with the rest of us?

Robert
 
Mind blowing knowledge you have in your head. Great talking to you again. Thanks for numbing my mind again. Going to have to find a way to plug a USB into my head for the next time we talk so I can absorb quicker. :rofl:
 
Thats cool. I have some experience with tissue culture. I have not grown peppers, but lots of papaya and many Euphorbiaceae (poinsettia and close relatives, as well as not-so-close relatives).

I have done embryo rescue as well, and have thought it would be a good idea to try to cross a pepper and a tomato to see if you could get a tomato that produces capsaicin.

I am very interested in the details of your grow. are you using MS medium? what hormones? and do you do your work in a glove box or do you have a hood? and what is your sterilization procedure?

Sorry for bombarding you with questions. I am really excited to see somebody doing this at home!

Thanks
 
Actually made my own sterile airflow hood out of a big tupperware on its side, a salvaged HEPA filter and a blower fan off a car. It works surprisingly well for what it is. Using MS + Agar solidifier, BAP & PPM along with a very light nutrient additive. Also propagating in the Unicorn jars, which are really better than the baby food jars.
So far I don't have a lot of experience with a bunch of different plants. Used to use a lot of Euphorbia on green roofs in the past - kinda funny when folks weeded them once without gloves and got the sap on their hands and arms :-)



Thats cool. I have some experience with tissue culture. I have not grown peppers, but lots of papaya and many Euphorbiaceae (poinsettia and close relatives, as well as not-so-close relatives).

I have done embryo rescue as well, and have thought it would be a good idea to try to cross a pepper and a tomato to see if you could get a tomato that produces capsaicin.

I am very interested in the details of your grow. are you using MS medium? what hormones? and do you do your work in a glove box or do you have a hood? and what is your sterilization procedure?

Sorry for bombarding you with questions. I am really excited to see somebody doing this at home!

Thanks
 
I'm doing another run on about 2 months to secure/stabilize a great line of Nardellos (from Justaguy!). Will document, picture and post the hell out of those when I do it again. Planning on doing a bunch this Fall as well.

can you post your recipe? hormone levels, etc?

and more pics?

thanks!
 
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